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KMID : 0359319990390010090
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Korean Journal of Veterinary Research
1999 Volume.39 No. 1 p.90 ~ p.95
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Detection of Mycoplasma gallisepticum using Polymerase Chain Reaction ( PCR )
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이영주/Lee, Young Ju
김종완/김기석/탁연빈/Kim, Jong Wan/Kim, Ki Seuk/Tak, Ryun Bin
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Abstract
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A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum (M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.
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KEYWORD
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